An internship using fish inner ear structures to estimate predation on salmon

Hi, my name is Reid Griffin, and I’m a 2023 Hollings Scholar. This summer, I studied the age and growth structures of smallmouth bass (Micropterus dolomieu) within the Weldon Headpond in Maine. My data contributed to a larger study on smallmouth bass predation of the endangered Atlantic salmon.

Reid, whose face is out of focus in the background, uses forceps to hold up a small white crystalline structure for the camera.
Reid holding a freshly extracted otolith, a structure from a fish’s inner ear.  (Image credit: Photo courtesy of Reid Griffin)

Purpose of the study

Atlantic salmon (Salmo salar) are endangered in the United States and have only one remaining wild U.S. population. The Weldon headpond is one of the most dangerous sites for smolts, or young salmon, in this population. One culprit behind higher death rates at Weldon Headpond may be predation by smallmouth bass. My study focused on understanding age and growth rates of smallmouth bass, which can help us better estimate predation in this area.

My project

We investigated smallmouth bass age and growth rates using a small calcium carbonate structure in their inner ear called otoliths. As the fish grows, so does the otolith. The otolith grows in a way that creates bands, similar to tree rings. You can count the bands to estimate the yearly age of a fish. In addition to that, the distance between bands also can tell us how much a fish has grown in that year. 

We extracted otoliths from roughly 50 smallmouth bass angled from the Weldon Headpond. To see the bands, you need to look at a thin cross section of the otolith under a microscope. We prepared the otoliths for imaging by cleaning them, cutting a thin cross section, and then polishing them using sandpaper to a very thin sliver. Once we counted and measured the bands, we estimated the fish’s average maximum size and the growth.

A microscope image focused on the semi-translucent otolith. The bottom of the otolith has a dark center core. Six dark bands with lighter color between them arc over the core. There are annotated measurement lines drawn across the otolith.
A microscope image of a sectioned otolith having its age and growth rates estimated. The annotations are used to measure and count the otolith core and bands. (Image credit: Reid Griffin)

Results

Once this was all done, we provided the data for use in a bioenergetics model. From the model, we can determine if smallmouth bass are statistically likely to be a major force behind the high Atlantic salmon death rates seen in the Weldon Headpond.